Differentiating organisms using a gram stain essay organisms using the gram stain introduction the experiment conducted was based upon the known attributes of two different groups of bacteria, those that are gram positive, and those that are gram negative. Gram stain permits the separation of all bacteria into two large groups, those which retain the primary dye (gram -positive) and those that take the color of the counterstain (gram -negative. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives the procedure is based on the ability of microorganisms to retain color of the stains used during the gram stain reaction. Gram staining is a method of differentiating bacterial species into two large groups (gram-positive and gram-negative) from: sterility, sterilisation and sterility assurance for pharmaceuticals , 2013. The gram stain differentiates two types of bacteria based on the composition of their cell walls it uses a primary stain, a mordant, a decolorizer, and a secondary stain to allow for the visualization of these structural differences.
This could make gram-negative organisms appear to be gram-positive or gram-variable take a fresh culture—old cultures stain erratically fix the cells to the slide by heat or by exposure to methanol. This is because they stain negative using the gram stain the gram stain is a differential technique that is commonly used for the purposes of classifying bacteria the staining technique distinguishes between two main types of bacteria (gram positive and gram negative) by imparting color on the cells. S pneumoniae can be identified using gram stain, catalase, and optochin tests simultaneously, with bile solubility as a confirmatory test if these tests indicate that the isolate is s pneumoniae , serological. Besides gram's stain, there is a wide range of staining methods available the procedures for these other methods follow quite closely those of gram's stain by using appropriate dyes, different parts of the cell structure such as capsules, flagella, granules, or spores can be stained.
Gram-staining is a differential staining technique that uses a primary stain and a secondary counterstain to distinguish between gram-positive and gram-negative bacteria table 1 gram stain process. This staining technique provides information about cell wall structure as gram-positive organisms have only a grid of peptidoglycan and gram-negative cells posses an additional lipid bilayer this information is an important predictor of reaction to antibiotics as many are only effective on gram-positive bacteria. The gram stain, developed by christian gram in the 1800's, was the first differential staining technique in use and is still an important tool for distinguishing between two main types of bacteria—gram-positive and gram-negative. • to learn the techniques of gram staining, nigrosin staining and koh test • to use and relate the gram stain to the study of bacterial cell morphology, and as an important step in the identification of a bacterial species.
Gram stain or gram staining, also called gram's method, is a method of staining used to distinguish and classify bacterial species into two large groups (gram-positive and gram-negative) the name comes from the danish bacteriologist hans christian gram , who developed the technique. Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents the gram stain procedure distinguishes between gram positive and gram negative groups by coloring these cells red or violet. Techniq ues application of a rapid method for gram differentiation of plant pathogenic and saprophytic bacteria without staining t v suslow, m n schroth, and m isaka. Amanda lab report 1 microbiology laboratory title - differentiating organisms using the gram stain introduction the experiment conducted was based upon the known attributes of two different groups of bacteria, those that are gram positive, and those that are gram negative.
A gram stain is a laboratory procedure used to detect the presence of bacteria and sometimes fungi in a sample taken from the site of a suspected infection it gives relatively quick results as to whether bacteria or fungi are present and, if so, the general type(s. Almost all bacteria can be differentiated by gram stain into the two groups whereas only a very small percentage of bacterial species are either spore formers or acid. The gram stain is the most important staining procedure in microbiology it is used to differentiate between gram positive organisms and gram negative organisms hence, it is a differential stain.
Gram-positive and gram-negative bacteria are classified based on the ability to retain the gram stain the gram-positive bacteria would retain the gram stain and observed as violet color after the application of iodine (as mordant) and alcohol (ethanol. Gram staining is based on the ability of bacteria cell wall to retaining the crystal violet dye during solvent treatment the cell walls for gram-positive microorganisms have a higher peptidoglycan and lower lipid content than gram-negative bacteria. Gram staining (or gram's method) is a method of differentiating bacterial species into two large groups (gram-positive and gram-negative) the name comes from its inventor, hans christian gram. Using a specific staining procedure, it is possible to differentiate the two types under a microscope the gram stain method of differentiation is possible because of differences in the cell membrane between the two categories of bacteria.
The developed dichotomous key separated the unknown samples into unknown a and unknown b by differentiating the organisms by their cell wall structure and morphology, using the gram stain procedure after separating the organism by whether they were gram-positive or gram-negative different test were suggested for each group separately. Some bacteria have walls of intermediate structure and, although they are officially classified as gram-positives because of their linage, they stain in a variable manner one prokaryote domain, the archaea, have such variability of wall structure that the gram stain is not a useful differentiating tool. Simple stains (eg methylene blue) are used to stain bacteria, differential stains (gram stain) are used to divide bacteria into different groups, acid-fast stains are used for mycobacterium sp and specific stains are used to stain cellular features such as negative stains for capsules, spore stains and flagellar stains. The gram stain the gram stain, originally developed in 1884 by christian gram, is probably the most important procedure in all of microbiology it has to be one of the most repeated procedures.