Example of a microbiology unknown lab report by taylor autry introduction in this paper i will discuss the processes of how i came to find my two unknown bacteria. Description and significance serratia marcescens is a motile,short rod-shaped, gram-negative, facultative anaerobe bacterium, classified as an opportunistic pathogen it was discovered in 1819 by bartolomeo bizio in padua, italy. Learn microbiology lab identification unknowns with free interactive flashcards choose from 500 different sets of microbiology lab identification unknowns flashcards on quizlet.
Unknown, it is customary to write, a trypticase soy agar (tsa) plate was used to isolate the unknown it is also customary to write in the past tense for most of the report. Lab report on unknown bacteria serratia marcescens in bacteria, temperature, ph, and other chemical agents all affect the expression of genes in this lab, the effect of temperature change on the gene which codes for a red pigment called prodigiosin of bacterium serratia marcescens is being tested. Serratia marcescens has a long history in the church, as well as in microbiologynumerous historical incidents recount that the blood red pigment produced by s marcescens growing in bread was interpreted as a sign of blood.
Abstract serratia marcescens, a gram-negative, rod-shaped, facultative anaerobe (fig 1), is ubiquitous in water, soil, and natural settingsit is easily grown in the lab and may serve as an ideal model for adaptation studies because of the natural color variation of s marcescens (gillen 2008. Serratia species are opportunistic gram-negative bacteria classified in the tribe klebsielleae and the large family enterobacteriaceae serratia are widespread in the environment, but are not a common component of the human fecal flora. August 23, 2013 tankeshwar bacteriology, culture media used in microbiology, laboratory diagnosis of bacterial disease, microbiology 14 eosin methylene blue agar is a both selective and differential culture medium.
Laboratory-acquired infections: 5 laboratory acquired infection with s marcescens have been reported as of 1999 footnote 11 sources/specimens : serratia spp are found in feces, wound exudates, respiratory specimen, blood, eye culture, and urine footnote 10. The laboratory component is a very important part of the microbiology course since it is here that you will learn to use the basic techniques of microbiology, which may be required of you by a potential employer. Guide to the identification of an unknown bacterium - methods and report format pg 1 a standard part of nearly all lab courses in introductory microbiology is an activity wherein the student must use everything that has been learned in the course to identify and. Serratia marcescens serratia marcescens is the most common species of serratia found in hospitals, and the only pathogenic species of serratia, except for rare reports of diseases resulting from infection with serratia plymuthica,serratia liquefaciens, serratia rubidaea, and serratia odorifera. Serratia marcescens by: amanda armbruster throughout this presentation i will formally walk you through the techniques, methods and steps in which i took over a four week period to find my unknown bacterium.
Color and almost fluorescent, and serratia marcescens was ruled out because it would have been pink (bergey et al 1989) after discovering that the unknown was gram negative, and examining. Any additional tests necessary to decipher the identity of your unknown perform these biochemical tests of unknown as part of the corresponding exercises in section 5 of the lab manual. Report for each of your unknown bacteria the template can be found linked to the online class syllabus these excel documents must also be linked to your electronic portfolio under the laboratory skills section.
The identification of bacteria #1 was very easy since there are few organisms which have a red growth, and serratia marcescens is the only one that is on our unknowns list when the gram stain was inconclusive for this bacterium, it was sensible to use other tests to positively identify it. Virtual laboratory report student: course: lab section 001 microbiology (tm) serratia marcescens, yersinia enterocolitica 9. On unknown sample 16 a support the conclusion that it is of the genus bacillus it is known that it is known that certain bacteria in the genus bacillus produce a variable gram stain result (microbe wiki.